Effect of Temperature and PH on Enzyme Lab Report

I’m working on a Biology question and need guidance to help me study.


Guidance for Enzyme Lab Report

This lab report will focus on your evaluation of how temperature and pH affect the rate of enzyme activity.

Introduction / Purpose (5 points)

  • Why did we do this lab?
  • Are there any important concepts or explanations that are relevant to the reader’s understanding of the purpose and background of the lab?
  • What is invertase? What chemical reaction does it catalyze? This can be a diagram.
  • What were your hypotheses for each of the experimental procedures we conducted?
  • What is Benedict’s test? How and why does it work? Why did we use it? What are reducing sugars?

Materials & Methods (5 points)

  • Follow the directions provided above regarding the level of detail to include here.
  • Convey the experimental design using a labeled diagram or image, if desired.

Results & Data (5 points)

  • Include any tables from the lab containing the data you collected
  • Include a written statement of the results of every single experiment you conducted.

Discussion & Conclusion (10 points)

  • Scientifically interpret your results for every experiment you conducted.
  • This means every temperature, and every pH.
  • Can you relate your results to Brownian motion or diffusion?
  • Can you relate your results to the effect of pH on secondary structure?
  • What does it actually mean when an enzyme is denatured by heat?
  • Say you obtained a negative result from Benedict’s test when you carried out the chemical reaction at the highest pH.
    • Why did you observe these results? You must provide an explanation that is based on your knowledge of enzymes, how they function, etc.
  • You may not completely understand why the invertase didn’t function in those conditions…it is your job to learn this.
  • You may have to reference your textbook or another reference to gain sufficient understanding of your experimental outcomes! 🙂


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