I’m trying to learn for my Biology class and I’m stuck. Can you help?

 

Fill in all parts and questions from the bottom half of p 4 and all of p 5, save in Word of PDF to your computer and upload to the Assignment.

I’m studying and need help with a Biology question to help me learn.

 

could you please help me with the discussion board, reading and answer the questions.

Need help with my Biology question – I’m studying for my class.

 

Yo will need to download the bio-rad software to do the assignment.

SDS-PAGE

Data can be found here: Gel Image.scn

Results should include:

Annotated gel figure with description (4 points)

• Each lane numbered
• Lanes containing the standard ladder labeled

Choose two lanes and calculate the molecular weight of each band in those lanes (empty lanes and standard lanes do not count). Report the MW of the bands in a table format. Indicate lane by its numbering in the gel figure. (4 points)

Discussion Points (3 points each):

1. Describe the spatial separation of differently sized proteins in SDS-PAGE. How does this compare to size exclusion chromatography?

2. Would this gel’s banding patterns be the same if SDS was not used? Why or why not?

3. How can looking at the standards test your claims in questions 1 and 2?

4. Discuss one challenge you faced in using the gel analysis software.

*For all discussion points, use the data to support your conclusions. One-sentence answers will not be considered discussions and will not receive full credit.*

PreviousNext

Here the tutorial link for image lab